Transposition-mediated DNA re-replication in maize
نویسندگان
چکیده
Every DNA segment in a eukaryotic genome normally replicates once and only once per cell cycle to maintain genome stability. We show here that this restriction can be bypassed through alternative transposition, a transposition reaction that utilizes the termini of two separate, nearby transposable elements (TEs). Our results suggest that alternative transposition during S phase can induce re-replication of the TEs and their flanking sequences. The DNA re-replication can spontaneously abort to generate double-strand breaks, which can be repaired to generate Composite Insertions composed of transposon termini flanking segmental duplications of various lengths. These results show how alternative transposition coupled with DNA replication and repair can significantly alter genome structure and may have contributed to rapid genome evolution in maize and possibly other eukaryotes.
منابع مشابه
Title: Transposition-mediated DNA re-replication in maize
1 Every DNA segment in a eukaryotic genome normally replicates once and only once per 2 cell cycle to maintain genome stability. We show here that this restriction can be bypassed 3 through alternative transposition, a transposition reaction that utilizes the termini of two 4 separate, nearby transposable elements (TEs). Our results suggest that alternative 5 transposition during S phase can in...
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A model of En transposition during chromosome replication is presented following a study of somatic events associated with the transposition of En in the endosperm tissue of the maize kernel. Two supporting assays, the excision and the postexcision events, were used in following these events. The excision of the En transposon has been monitored in the starch-producing endosperm tissue by using ...
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In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids ("chromatid selectivity"). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethy...
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عنوان ژورنال:
دوره 3 شماره
صفحات -
تاریخ انتشار 2014